Review



bmpr2 coding sequence  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 91
      Buy from Supplier

    Structured Review

    Addgene inc bmpr2 coding sequence
    Figure 1. The BMP pathway in the colon. BMP2 and BMP4 are the most abundant ligands of the BMP pathway in the bowel mucosa. They are able to bind the <t>BMPR1A-BMPR2</t> complex on the surface of colonocytes, therefore, triggering R-SMAD phosphorylation and downstream gene regulation in the canonical pathway. In addition, BMP can also mediate proliferation by convergent pathways, such as ERK, JNK, and p38. Created with BioRender.com.
    Bmpr2 Coding Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bmpr2 coding sequence/product/Addgene inc
    Average 91 stars, based on 1 article reviews
    bmpr2 coding sequence - by Bioz Stars, 2026-05
    91/100 stars

    Images

    1) Product Images from "BMPR2 as a Novel Predisposition Gene for Hereditary Colorectal Polyposis."

    Article Title: BMPR2 as a Novel Predisposition Gene for Hereditary Colorectal Polyposis.

    Journal: Gastroenterology

    doi: 10.1053/j.gastro.2023.03.006

    Figure 1. The BMP pathway in the colon. BMP2 and BMP4 are the most abundant ligands of the BMP pathway in the bowel mucosa. They are able to bind the BMPR1A-BMPR2 complex on the surface of colonocytes, therefore, triggering R-SMAD phosphorylation and downstream gene regulation in the canonical pathway. In addition, BMP can also mediate proliferation by convergent pathways, such as ERK, JNK, and p38. Created with BioRender.com.
    Figure Legend Snippet: Figure 1. The BMP pathway in the colon. BMP2 and BMP4 are the most abundant ligands of the BMP pathway in the bowel mucosa. They are able to bind the BMPR1A-BMPR2 complex on the surface of colonocytes, therefore, triggering R-SMAD phosphorylation and downstream gene regulation in the canonical pathway. In addition, BMP can also mediate proliferation by convergent pathways, such as ERK, JNK, and p38. Created with BioRender.com.

    Techniques Used: Phospho-proteomics

    Figure 2. BMPR2 variants drive a proliferative response and impair the BMP4-mediated growth inhibition. Characterization of the proliferative capacity of 2 different BMPR2 clones (B26 and B31) in which the 3 BMPR2 variants were reintroduced. (A) MTS cell proliferation assay. Samples were assayed in triplicate and the experiment was repeated 5 times (n ¼ 5). (B) Plating efficiency (colonies originated from single cells) in the colony-formation assay. Samples were assayed in duplicate and the experiment was repeated 3 times (n ¼ 3). (C) MTS cell proliferation assay in the presence or absence of 50 ng/mL of BMP4. Data are represented as the growth difference between treated and untreated cells. Samples were assayed in triplicate and the experiment was repeated 4 times (n ¼ 4). Data represent mean ± SD. *P < .05, **P < .01, ***P < .001 for the analysis of variance with the least significant difference post-hoc test.
    Figure Legend Snippet: Figure 2. BMPR2 variants drive a proliferative response and impair the BMP4-mediated growth inhibition. Characterization of the proliferative capacity of 2 different BMPR2 clones (B26 and B31) in which the 3 BMPR2 variants were reintroduced. (A) MTS cell proliferation assay. Samples were assayed in triplicate and the experiment was repeated 5 times (n ¼ 5). (B) Plating efficiency (colonies originated from single cells) in the colony-formation assay. Samples were assayed in duplicate and the experiment was repeated 3 times (n ¼ 3). (C) MTS cell proliferation assay in the presence or absence of 50 ng/mL of BMP4. Data are represented as the growth difference between treated and untreated cells. Samples were assayed in triplicate and the experiment was repeated 4 times (n ¼ 4). Data represent mean ± SD. *P < .05, **P < .01, ***P < .001 for the analysis of variance with the least significant difference post-hoc test.

    Techniques Used: Inhibition, Clone Assay, Proliferation Assay, Colony Assay

    Figure 3. BMPR2 variants act through SMAD or non-SMAD effectors, depending on their position. Characterization of the SMAD1/5/8 pathway activity of 2 different BMPR2 clones (B26 and B31) in which the 3 BMPR2 variants were reintroduced. (A) Quantitative analysis of the phosphorylated levels of SMAD1 (Ser463/465) by means of enzyme-linked immunosorbent assay in the presence or absence of 50 ng/mL of BMP4. Samples were assayed in duplicate and the experiment was repeated 3 times (n ¼ 3). (B) Real-time PCR quantification of ID1 and ID3 expression levels, 2 canonical BMP-SMAD downstream targets, in clone B26. Samples were assayed in triplicate and the experiment was repeated 3 times (n ¼ 3). Data represent mean ± SD. *P < .05, **P < .01, ***P < .001 for the analysis of variance with the least significant difference post-hoc test.
    Figure Legend Snippet: Figure 3. BMPR2 variants act through SMAD or non-SMAD effectors, depending on their position. Characterization of the SMAD1/5/8 pathway activity of 2 different BMPR2 clones (B26 and B31) in which the 3 BMPR2 variants were reintroduced. (A) Quantitative analysis of the phosphorylated levels of SMAD1 (Ser463/465) by means of enzyme-linked immunosorbent assay in the presence or absence of 50 ng/mL of BMP4. Samples were assayed in duplicate and the experiment was repeated 3 times (n ¼ 3). (B) Real-time PCR quantification of ID1 and ID3 expression levels, 2 canonical BMP-SMAD downstream targets, in clone B26. Samples were assayed in triplicate and the experiment was repeated 3 times (n ¼ 3). Data represent mean ± SD. *P < .05, **P < .01, ***P < .001 for the analysis of variance with the least significant difference post-hoc test.

    Techniques Used: Activity Assay, Clone Assay, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction, Expressing



    Similar Products

    bmpr2 coding sequence  (Addgene inc)
    91
    Addgene inc bmpr2 coding sequence
    Figure 1. The BMP pathway in the colon. BMP2 and BMP4 are the most abundant ligands of the BMP pathway in the bowel mucosa. They are able to bind the <t>BMPR1A-BMPR2</t> complex on the surface of colonocytes, therefore, triggering R-SMAD phosphorylation and downstream gene regulation in the canonical pathway. In addition, BMP can also mediate proliferation by convergent pathways, such as ERK, JNK, and p38. Created with BioRender.com.
    Bmpr2 Coding Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bmpr2 coding sequence/product/Addgene inc
    Average 91 stars, based on 1 article reviews
    bmpr2 coding sequence - by Bioz Stars, 2026-05
    91/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 1. The BMP pathway in the colon. BMP2 and BMP4 are the most abundant ligands of the BMP pathway in the bowel mucosa. They are able to bind the BMPR1A-BMPR2 complex on the surface of colonocytes, therefore, triggering R-SMAD phosphorylation and downstream gene regulation in the canonical pathway. In addition, BMP can also mediate proliferation by convergent pathways, such as ERK, JNK, and p38. Created with BioRender.com.

    Journal: Gastroenterology

    Article Title: BMPR2 as a Novel Predisposition Gene for Hereditary Colorectal Polyposis.

    doi: 10.1053/j.gastro.2023.03.006

    Figure Lengend Snippet: Figure 1. The BMP pathway in the colon. BMP2 and BMP4 are the most abundant ligands of the BMP pathway in the bowel mucosa. They are able to bind the BMPR1A-BMPR2 complex on the surface of colonocytes, therefore, triggering R-SMAD phosphorylation and downstream gene regulation in the canonical pathway. In addition, BMP can also mediate proliferation by convergent pathways, such as ERK, JNK, and p38. Created with BioRender.com.

    Article Snippet: The identified BMPR2 variants were introduced into the BMPR2 coding sequence (no. 116718, Addgene) by a simple 1- step PCR amplification with nonoverlapping primers (Q5 SiteDirected Mutagenesis Kit, New England Biolabs, Ipswich, MA).

    Techniques: Phospho-proteomics

    Figure 2. BMPR2 variants drive a proliferative response and impair the BMP4-mediated growth inhibition. Characterization of the proliferative capacity of 2 different BMPR2 clones (B26 and B31) in which the 3 BMPR2 variants were reintroduced. (A) MTS cell proliferation assay. Samples were assayed in triplicate and the experiment was repeated 5 times (n ¼ 5). (B) Plating efficiency (colonies originated from single cells) in the colony-formation assay. Samples were assayed in duplicate and the experiment was repeated 3 times (n ¼ 3). (C) MTS cell proliferation assay in the presence or absence of 50 ng/mL of BMP4. Data are represented as the growth difference between treated and untreated cells. Samples were assayed in triplicate and the experiment was repeated 4 times (n ¼ 4). Data represent mean ± SD. *P < .05, **P < .01, ***P < .001 for the analysis of variance with the least significant difference post-hoc test.

    Journal: Gastroenterology

    Article Title: BMPR2 as a Novel Predisposition Gene for Hereditary Colorectal Polyposis.

    doi: 10.1053/j.gastro.2023.03.006

    Figure Lengend Snippet: Figure 2. BMPR2 variants drive a proliferative response and impair the BMP4-mediated growth inhibition. Characterization of the proliferative capacity of 2 different BMPR2 clones (B26 and B31) in which the 3 BMPR2 variants were reintroduced. (A) MTS cell proliferation assay. Samples were assayed in triplicate and the experiment was repeated 5 times (n ¼ 5). (B) Plating efficiency (colonies originated from single cells) in the colony-formation assay. Samples were assayed in duplicate and the experiment was repeated 3 times (n ¼ 3). (C) MTS cell proliferation assay in the presence or absence of 50 ng/mL of BMP4. Data are represented as the growth difference between treated and untreated cells. Samples were assayed in triplicate and the experiment was repeated 4 times (n ¼ 4). Data represent mean ± SD. *P < .05, **P < .01, ***P < .001 for the analysis of variance with the least significant difference post-hoc test.

    Article Snippet: The identified BMPR2 variants were introduced into the BMPR2 coding sequence (no. 116718, Addgene) by a simple 1- step PCR amplification with nonoverlapping primers (Q5 SiteDirected Mutagenesis Kit, New England Biolabs, Ipswich, MA).

    Techniques: Inhibition, Clone Assay, Proliferation Assay, Colony Assay

    Figure 3. BMPR2 variants act through SMAD or non-SMAD effectors, depending on their position. Characterization of the SMAD1/5/8 pathway activity of 2 different BMPR2 clones (B26 and B31) in which the 3 BMPR2 variants were reintroduced. (A) Quantitative analysis of the phosphorylated levels of SMAD1 (Ser463/465) by means of enzyme-linked immunosorbent assay in the presence or absence of 50 ng/mL of BMP4. Samples were assayed in duplicate and the experiment was repeated 3 times (n ¼ 3). (B) Real-time PCR quantification of ID1 and ID3 expression levels, 2 canonical BMP-SMAD downstream targets, in clone B26. Samples were assayed in triplicate and the experiment was repeated 3 times (n ¼ 3). Data represent mean ± SD. *P < .05, **P < .01, ***P < .001 for the analysis of variance with the least significant difference post-hoc test.

    Journal: Gastroenterology

    Article Title: BMPR2 as a Novel Predisposition Gene for Hereditary Colorectal Polyposis.

    doi: 10.1053/j.gastro.2023.03.006

    Figure Lengend Snippet: Figure 3. BMPR2 variants act through SMAD or non-SMAD effectors, depending on their position. Characterization of the SMAD1/5/8 pathway activity of 2 different BMPR2 clones (B26 and B31) in which the 3 BMPR2 variants were reintroduced. (A) Quantitative analysis of the phosphorylated levels of SMAD1 (Ser463/465) by means of enzyme-linked immunosorbent assay in the presence or absence of 50 ng/mL of BMP4. Samples were assayed in duplicate and the experiment was repeated 3 times (n ¼ 3). (B) Real-time PCR quantification of ID1 and ID3 expression levels, 2 canonical BMP-SMAD downstream targets, in clone B26. Samples were assayed in triplicate and the experiment was repeated 3 times (n ¼ 3). Data represent mean ± SD. *P < .05, **P < .01, ***P < .001 for the analysis of variance with the least significant difference post-hoc test.

    Article Snippet: The identified BMPR2 variants were introduced into the BMPR2 coding sequence (no. 116718, Addgene) by a simple 1- step PCR amplification with nonoverlapping primers (Q5 SiteDirected Mutagenesis Kit, New England Biolabs, Ipswich, MA).

    Techniques: Activity Assay, Clone Assay, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction, Expressing